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Production of Colony Stimulating Activity in Mixed Mononuclear Cell Culture
Author(s) -
Hellmann Andrzej,
Th'ng K. H.,
Goldman John M.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb07144.x
Subject(s) - peripheral blood mononuclear cell , phytohaemagglutinin , progenitor cell , cfu gm , granulocyte , bone marrow , andrology , immunology , incubation , agar , granulocyte colony stimulating factor , peripheral blood , microbiology and biotechnology , chemistry , medicine , biology , in vitro , stem cell , biochemistry , chemotherapy , genetics , bacteria
S ummary Culture medium was harvested after co‐incubation of mononuclear cells collected and pooled from the peripheral blood of two different normal donors and was tested for colony‐stimulating activity (CSA) in agar culture. With bone marrow from normal donors or peripheral blood from patients with chronic granulocytic leukaemia as sources of granulocyte‐committed progenitor cells (CFU‐c), such mixed mononuclear cell conditioned medium (MMC‐CM) showed activity equal to that of unfractionated leucocyte feeder layers and greater than that of CSA prepared from lymphocytes stimulated by phytohaemagglutinin. The addition to plates of 2‐mercaptoethanol during the preparation of MMC‐CM enhanced CSA release. MMC‐CM is thus a convenient source of CSA and its use may be preferable to that of feeder layers when day‐to‐day reproducibility is essential.