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The Synthesis of Folate‐binding Protein in Lymphocytes during Transformation
Author(s) -
Costa Maria da,
Sharon Michael
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb06014.x
Subject(s) - phytohaemagglutinin , protein biosynthesis , rna , antiserum , biochemistry , transformation (genetics) , radioimmunoassay , intracellular , immunoprecipitation , binding protein , chemistry , microbiology and biotechnology , biology , antibody , immunology , in vitro , gene
S ummary . The blast transformation of human lymphocytes exposed to phytohaemagglutinin was associated with an increase in the intracellular concentration of a folate‐binding protein. The protein was measured by a radioinimunoassay and by immunofluorescent microscopy using an antiserum raised against purified folatebinding protein prepared from chronic myelogenous leukaemia cells. Because it has been previously shown that protein‐bound folate cannot function as a cofactor or substrate, the synthesis of this binder during some phase of the replicative cycle may serve to modulate folate‐dependent RNA and DNA synthesis by controlling the concentration of free folate.