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Marrow Cells from Patients with Untreated Pernicious Anaemia Cannot Use Tetrahydrofolate Normally
Author(s) -
Deacon Rosemary,
Chanarin I.,
Perry Janet,
Lumb Michael
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb06008.x
Subject(s) - cobalamin , deoxyuridine , pernicious anaemia , bone marrow , medicine , vitamin b12 , gastroenterology , immunology , biochemistry , chemistry , dna
S ummary . Folate analogues were added to human bone marrow cells to determine their effect on deoxyuridine utilization in the deoxyuridine suppression test. Forniyltetrahydrofolates fully corrected the impairment of dU utilization in pernicious anaemia marrows but tetrahydrofolate was relatively ineffective. All these analogues were effective in megaloblastic marrows from folate deficient patients. Formyltetrahydrofolates also enhanced dU utilization by normal human marrows whereas methyltetrahydrofolate reduced its use. In terms of the methylfolate trap hypothesis, the expectation that cobalamindeficient marrows would be able to use tetrahydrofolate normally was not realized.