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The Abilities of Human Blood Platelets to Bind Extracellular Calcium and to be Aggregated by Adenosine Diphosphate are Related
Author(s) -
Taylor Patricia M.,
Heptinstall S.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb05941.x
Subject(s) - platelet , calcium , extracellular , chemistry , adenosine diphosphate , biochemistry , platelet rich plasma , biophysics , adenosine , platelet aggregation , medicine , biology , organic chemistry
S ummary . A technique is described for preparing platelet rich plasma containing a very low level of extracellular calcium. Using this Ca‐depleted PRY it has been possible to demonstrate that extraplatelet calcium is in equilibrium with a small amount of calcium that is bound to the platelet surface. When Ca‐depleted PRP is incubated at 37°C the platelets lose their ability to bind calcium. At the same time they lose their ability to aggregate. It is likely that the binding of calcium to the platelet surface is a prerequisite for ADP‐induced aggregation.