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Studies on Circulating Soluble Fibrin: Separation of 125 I‐Des‐AB Fibrin and 131 I‐Fibrinogen by Gel Filtration
Author(s) -
Kröhnke Isa,
Mahn I.,
Krell W.,
MüixerBerghaus G.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb03818.x
Subject(s) - fibrin , fibrinogen , chemistry , size exclusion chromatography , chromatography , thrombin , urea , biochemistry , platelet , immunology , enzyme , biology
S ummary . The behaviour of labelled des‐AB fibrin in plasma was studied by gel filtration after it had been injected into rabbits. Purified rabbit [ 125 I]des‐AB fibrin was prepared by clotting of [ 125 I]fibrinogen by thrombin and solubilizing the formed clot in buffered 3 M urea. Gel filtration of this material on urea‐equilibrated columns showed a single peak identical to the elution profile of fibrinogen. This indicated the existence of monomeric des‐AB fibrin. When plasma from rabbits injected with monomeric [ ,25 I]des‐AB fibrin and [ ,31 I]fibrinogen was gel‐filtered through plasma‐equilibrated columns, two separate peaks of radioactivities were obtained. The first peak eluted mainly with the void volume and contained [ I25 I]des‐AB fibrin whereas the second peak eluting within the fractionation range contained [ 131 I]fibrinogen. Identical elution profiles were obtained in in vitro studies when monomeric [ 125 I]des‐AB fibrin was mixed with plasma containing [ 131 I]fibrinogen and gel‐filtered through plasma‐equilibrated columns. We conclude from these studies that monomeric des‐AB fibrin formed high‐molecular weight aggregates or changed its conformation posing as a larger molecule than fibrinogen when injected into rabbits. No complex formation between des‐AB fibrin and fibrinogen was observed as [ 131 I]fibrinogen was not incorporated into des‐AB fibrin aggregates. Thus, soluble des‐AB fibrin can circulate in the blood without forming fibrin–fibrinogen complexes.