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A Factor VII Concentrate for Therapeutic Use
Author(s) -
Dike G. W. R.,
Griffiths D.,
Bidwell E.,
Snape T.J.,
Rizza C. R.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb03816.x
Subject(s) - cryoprecipitate , chromatography , chemistry , factor vii , fractionation , factor ix , human plasma , adsorption , medicine , biochemistry , organic chemistry , fibrinogen , coagulation
S ummary . A concentrate of factor VII suitable for therapeutic use has been prepared from human plasma by a method forming part of a comprehensive scheme of large‐scale plasma fractionation. Factor VIII was separated as cryoprecipitate and factors II, IX and X were adsorbed on DEAE‐cellulose. Most of the factor VII remained in the supernatant. By batch adsorption on DEAE‐Sepharose, followed by elution on a chromatographic column, factor VII was concentrated about 25‐fold, and purified about 50‐fold compared with original plasma, without the need for further dialysis or concentration steps. Data are presented from 10 batches, each from 80‐120 kg plasma. Following doses of factor VII to six congenitally deficient patients, the mean rise in plasma factor VII was 95–100% of theoretical; the half‐disappearance time was about 4 h. The treatment of four patients with acquired deficiency of factor VII is also described. No untoward side effects were observed.