Premium
Determination of Alpha Thalassaemia Phenotypes by Messenger RNA Analysis
Author(s) -
Hunt D. M.,
Higgs D. R.,
Old J. M.,
Clegg J. B.,
Weatherall D.J.,
Marsh G. W.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb03810.x
Subject(s) - phenotype , messenger rna , alpha (finance) , thalassemia , rna , genetics , biology , computational biology , medicine , gene , surgery , construct validity , patient satisfaction
S ummary . The possibility of using α/β globin messenger RNA (mRNA) ratios to distinguish between the carrier states for different forms of a thalassaemia has been explored, α/β globin mRNA ratios were determined in the red cells of a series of normal individuals and in members of four Cypriot families, one Thai and one Chinese family in which at least one person has haemoglobin H disease. It was found that there was a clear distinction in the ratios between normals, α thalassaemia 1 carriers, α thalassaemia 2 carriers, and those with haemoglobin H disease. This method should be a valuable addition to haematological analysis, haemoglobin synthesis and restriction mapping of DNA for the further elucidation of the genetics of α thalassaemia.