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An Agarose Plate Method for Detecting Alloantisera to Coagulant Factor IX and Factor IX Antigen
Author(s) -
Reisner Howard M.,
Strand Elizabeth A.,
Chung KuoSan,
Roberts Harold R.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb01214.x
Subject(s) - agarose , factor ix , coagulation , haemophilia b , haemophilia , antiserum , antigen , sepharose , microbiology and biotechnology , chemistry , haemophilia a , immunology , medicine , chromatography , biochemistry , biology , enzyme , surgery
S ummary . An agarose plate method for detecting human alloantibodies (inhibitors) to coagulant factor IX has been developed. The assay is based on the ability of such antisera to inhibit the coagulation of a mixture of haemophilia B plasma, normal plasma and platelet substitute in an agarose matrix. The agarose plate method was also adopted to measure levels of FIX antigen (IX:Ag) in plasma. Using this technique, 12 of 17 obligate carriers of haemophilia B demonstrated reduced levels of IX:Ag. Three of the five carriers with normal IX:Ag levels were members of kindred in which affected individuals had normal or near normal levels of IX:Ag.