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Characterization of Null Cells in Chronic Lymphocytic Leukaemia with B‐Cell Allo‐ and Hetero‐antisera
Author(s) -
Kirov Sylvia M.,
Kwant W. O.,
Fernandez L. A.,
MacSween J. M.,
Langley G. R.
Publication year - 1980
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1980.tb01205.x
Subject(s) - null cell , antiserum , biology , antigen , population , b cell , microbiology and biotechnology , cytotoxic t cell , immunology , peripheral blood mononuclear cell , lymphocyte , lymphoblast , cell culture , antibody , in vitro , medicine , genetics , environmental health
S ummary . Chronic lymphocytic leukaemia peripheral blood mononuclear cells (CLL‐PBMN) were separated into B, T and Null‐enriched lymphocyte sub‐populations using sequential mouse and sheep red blood cell rosetting depletions on Hypaque–Ficoll gradients. The procedure produced viable cell populations with mean percentage purities of 90, 87 and 75 for B, T and non‐rosetting (Null‐enriched) sub‐populations, respectively. More than 80% of PBMN cells were generally accounted for by mouse and sheep rosetting. The purified lymphocyte sub‐populations were examined with a panel of B‐cell specific alloantisera obtained from kidney transplant recipients and a rabbit antiserum to B cell antigen isolated from a human B‐lymphoblastoid line. The results illustrated that the antigens detected by these sera also have potential as a marker for characterizing the CLL population. Where conventional markers were weak or absent, B cell antigens were readily detected in both fluorescent and cytotoxic tests. The majority of the non‐rosetting cells (> 90%) in CLL followed similar patterns of reactivity to the purified B cells, suggesting they are a subset of B cells. A small residual population (0–5% of PBMN) did not react with the antisera, the significance of which is unknown.