Premium
An Examination of Complement Proteins on Membranes of Paroxysmal Nocturnal Haemoglobinuria (PNH) and PNH‐like Red Cells
Author(s) -
Burapakulsolsri Noppaporn,
Yuthavong Yongyuth,
Wilairat Prapon
Publication year - 1979
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1979.tb05872.x
Subject(s) - cd59 , medicine , complement (music) , erythrocyte membrane , paroxysmal nocturnal hemoglobinuria , immunology , complement system , membrane , chemistry , antibody , biochemistry , phenotype , gene , complementation
S ummary . Proteins obtained from membranes of PNH erythrocytes and of red cells treated with 2‐aminoethylisothiouronium bromide (AET) following lysis in acidified serum (Ham's test) were compared by sodium dodecylsulphate‐polyacrylamide gel electrophoresis. The peptide patterns were identical, providing support that AET‐treated red cells afford a suitable experimental substitute for PNH red cells in studies involving complement fixation.