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The Separate Effects of H + and 2,3‐DPG on the Oxygen Equilibrium Curve of Human Blood
Author(s) -
Samaja M.,
Winslow R. M.
Publication year - 1979
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1979.tb05870.x
Subject(s) - diphosphoglycerate , oxygen , chemistry , allosteric regulation , oxygen–haemoglobin dissociation curve , saturation (graph theory) , p50 , extracellular , bohr effect , intracellular , oxygen saturation , biochemistry , mathematics , receptor , combinatorics , transcription factor , gene , organic chemistry
S ummary . Addition of non‐saturating amounts of 2,3‐DPG (2,3‐diphosphoglycerate) within the red cell (2,3‐DPG/haemoglobin less than I) intially reduces Hill's parameter, n. With increasing 2,3‐DPG/haemoglobin, n increases until a maximum is reached at 2,3‐DPG/haemoglobin greater than I. Thus, 2,3‐DPG influences the shape as well as the position of the whole blood oxygen equilibrium curve (OEC). The importance of this effect on the oxygen carrying capacity of the blood is considered. The effect of 2,3‐DPG on the position of the OEC (p50, the pO 2 at one‐half maximal O 2 saturation) is via its allosteric effect on haemoglobin at 2,3‐DPG/Haemoglobin less than I. Above that ratio, its effect is to reduce intracellular relative to the extracellular pH.