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Variability of Intracellular pH within Individual Populations of SS and AA Erythrocytes
Author(s) -
Kaperonis A. A.,
Bertles J. F.,
Chien S.
Publication year - 1979
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1979.tb03766.x
Subject(s) - intracellular , chemistry , centrifugation , membrane , red blood cell , in vivo , mole , intracellular ph , redistribution (election) , biophysics , differential centrifugation , biochemistry , biology , genetics , politics , political science , law
S ummary . Individual populations of AA and SS erythrocytes were fractionated according to cell density by centrifugation, and the fractions analysed for intracellular pH (pH i ), the mole ratio of 2,3‐diphosphoglycerate to haemoglobin (DPG:Hb), and cell concentration of haemoglobin (MCHC). The pH i of SS erythrocytes was consistently lower than that of AA erythrocytes throughout the density range, and the lowest pH i of both cell types (AA and SS) was found in cells with the highest density. As the highest density AA and SS erythrocytes are characterized by the lowest DPG:Hb values, their relatively low pH i cannot be ascribed to intracellular organic phosphate. Instead we propose that a redistribution of hydrogen ions across the membranes of both AA and SS erythrocytes is the ultimate result of progressive alterations in these membranes in vivo.