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Reduced Granulocyte‐Macrophage Colony‐Stimulating Activity by Mitogen‐Stimulated Lymphocytes from Patients with Aplastic Anaemia
Author(s) -
Weatherly Thomas L.,
Fleisher Thomas A.,
Strong Douglas M.
Publication year - 1979
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1979.tb03760.x
Subject(s) - phytohaemagglutinin , peripheral blood mononuclear cell , immunology , peripheral blood , medicine , granulocyte , lymphocyte , aplastic anemia , macrophage , mitogen activated protein kinase , biology , bone marrow , in vitro , biochemistry
S ummary . The ability of peripheral blood mononuclear cells from patients with idiopathic aplastic anaemia to provide colony‐stimulating activity (CSA) was compared to that of normal controls. CSA was prepared by incubating peripheral blood mononuclear cells with phytohaemagglutinin. The supernatant derived from the latter is known as activated lymphocyte‐conditioned medium (ALCM). The CSA of ALCM in eight patients was compared to that of normals in 12 experiments. In all but one instance there was decreased CSA by patient ALCM. Possible implications of these findings are discussed.