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ADCC (K‐Cell) Lysis of Human Erythrocytes Sensitized with Rhesus Alloantibodies
Author(s) -
Urbaniak S. J.
Publication year - 1979
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1979.tb01135.x
Subject(s) - lysis , antibody dependent cell mediated cytotoxicity , phagocytosis , antibody , microbiology and biotechnology , biology , peripheral blood mononuclear cell , chemistry , immunology , biochemistry , in vitro , monoclonal antibody
S ummary . The mechanism of lysis of anti‐D coated human erythrocytes by human mononuclear K‐cells was investigated. Red cell lysis was measureable after 30 min incubation and reached a maximum by 18–20 h. Cell‐to‐cell contact was necessary for lysis, phagocytosis was not a prerequisite, and intact microfilament function was required. The divalent cations Ca 2+ and Mg 2+ were both required for lysis to occur. Studies with metabolic inhibitors indicate that some RNA and protein synthesis is required for maximum expression of ADCC and intact microtubule function is essential. In the present system lysis was mediated by IgG 1 anti‐D antibodies and was signficantly inhibited by IgG 1 and IgG 3 subclasses with some inhibition by IgG 2 but not by IgG 4 , IgA or IgM. This suggests that the K‐cell Fc receptor is specific for IgG but that there is major cross‐reactivity between IgG 1 and IgG 3 . The inhibiting effect of hydrocortisone suggests that ADCC inhibition may be one mode of action of corticosteroids in ameliorating autoimmune haemolytic anaemia.