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Ferritin as a Cytosol Iron Transport Intermediate in Human Reticulocytes
Author(s) -
Speyer Barbara E.,
Fielding J.
Publication year - 1979
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1979.tb01130.x
Subject(s) - ferritin , reticulocyte , cytosol , incubation , transferrin , biochemistry , chemistry , biology , enzyme , messenger rna , gene
S ummary . The major iron‐bearing cytosol components of human reticulocytes identified after incubation with 59 Fe– 125 I‐transferrin have been studied further. Component C previously found to behave consistently as an intermediate in the iron transport pathway to haem is shown to consist entirely of ferritin. After a short pulse of labelled transferrin incubation, chase experiments showed a fall of ferritin label with time and a corresponding increase in haemoglobin‐iron incorporation. There was no loss of ferritin to the culture medium. Restriction of iron uptake by reticulocytes using both p‐hydroxymercuribenzoate inhibition of uptake and incubation with progressively lower saturations of iron‐transferrin gave linearly related incorporation of 59 Fe into ferritin and haemoglobin at all levels of iron uptake, thus negating the concept of ferritin as an ‘overspill’ form of reticulocyte iron. The results suggest that cytosol ferritin is an obligatory intermediate in reticulocyte iron transport.