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Characterization of Serum Fibrinogen and Fibrin Fragments Produced during Disseminated Intravascular Coagulation
Author(s) -
Lane D. A.,
Preston F. E.,
Ross M. E.,
Kakkar V. V.
Publication year - 1978
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1978.tb05837.x
Subject(s) - fibrinogen , fibrin , plasmin , disseminated intravascular coagulation , factor xiiia , chemistry , thrombin , coagulation , lysis , factor xiii , gel electrophoresis , polyacrylamide gel electrophoresis , biochemistry , microbiology and biotechnology , platelet , immunology , pathology , medicine , biology , enzyme
S ummary . The fibrinogen and fibrin degradation products (FDP) in serum samples taken from nine patients with suspected disseminated intravascular coagulation have been characterized using a method of immunoprecipitation followed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. All of the serum samples contained a fragment with the same electrophoretic mobility as fibrinogen fragment X, while the majority also had evidence of fragments with similar mobility to fibrinogen fragments Y and D. In eight of the nine serum samples there was strong evidence of the D‐dimer fragment that is released by plasmin lysis of crosslinked fibrin. Also present in all but one of the samples were fragments of higher molecular weight than fibrinogen which were probably soluble, non‐clottable, factor XIIIa induced crosslinked derivatives of fibrinogen. These results suggest that during disseminated intravascular coagulation thrombin and activated factor XIII act upon fibrin(ogen) to form complexes that are subsequently lysed by plasmin to produce soluble crosslinked derivatives of fibrin.