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Organelle Pathology in Primary and Secondary Haemochromatosis with Special Reference to Lysosomal Changes
Author(s) -
Seymour Carol A.,
Peters T. J.
Publication year - 1978
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1978.tb03661.x
Subject(s) - peroxisome , organelle , endoplasmic reticulum , pathology , bone canaliculus , mitochondrion , hemochromatosis , cell fractionation , population , biology , liver biopsy , microbiology and biotechnology , biopsy , biochemistry , medicine , enzyme , genetics , environmental health , gene
S ummary . The organelle pathology of liver biopsy specimens from patients with either primary or secondary haemochromatosis was investigated by analytical subcellular fractionation in combination with enzymic microanalysis. The most striking changes were found in the lysosomes. Increased total activities but decreased latent activities of enzymes selectively localized to the high density population of lysosomes was demonstrated in the iron overloaded biopsies. Depletion of the iron, where possible, by venesection was accompained by a return to normal of these changes. The other subcellular organelles, plasma membrane, endoplasmic reticulum, biliary canaliculi, mitochondria, peroxisomes and the low density population of lysosomes appear to be relatively unaffected. The minor changes demonstrated are similar to those seen in other forms of chronic liver disease. It is suggested that iron mediated lysosomal disruption is implicated in the pathogenesis of the tissue damage in haemochromatosis.