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Stimulation of in Vitro Granulocyte‐Macrophage Colony Formation by Mouse Heart Conditioned Medium
Author(s) -
Byrne Peter V.,
Heit Wolfgang,
Kubanek Bernhard
Publication year - 1978
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1978.tb03657.x
Subject(s) - differential centrifugation , in vitro , stimulation , granulocyte , colony stimulating factor , biology , macrophage , bone marrow , granulocyte macrophage colony stimulating factor , microbiology and biotechnology , immunology , andrology , endocrinology , biochemistry , medicine , haematopoiesis , stem cell
S ummary . Medium conditioned by mosue heart tissue was found to contain colony‐stimulating factor(s) (CSF) which prodduced in vitro colonies of granulocytes and/or macrophages with bone marrow cells of CBA mice. The action of this CSF type (CSF MHCM ) was compared with that of post‐endotoxin mouse serum (CSF ES ) on colony formation by density subpopulations of colony‐forming cells (CFU‐c), separated by isopycnic centrifugation in a continous albumin density gradient. The number, size and morphology of colonies were examined. Mouse heart conditioned medium produced slightly more colonies adn of larger size than endotoxin serum but the density distribution of their CFU‐c were similar. Low density CFU‐c produced predominatly pure granulocytic colonies and high density ones predominately pure macrophage colonies with both types of CSF. There was no apparent relationship between colony size and CFU‐c density.