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Characterization of Marrow Granulocyte Development: Changes in Response to Inflammatory Reactions
Author(s) -
Scott R. B.,
Eanes R. Z.,
Cooper Laverne W.,
Higgins Linda L.,
Eastment Christine A.
Publication year - 1977
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1977.tb01023.x
Subject(s) - granulocyte , bone marrow , biology , ficoll , granule (geology) , elutriation , lymphocyte , immunology , chemistry , biochemistry , in vitro , peripheral blood mononuclear cell , paleontology , organic chemistry
S ummary . Studies of marrow granulocyte differentiation require separation of precursors into populations of varying stages of maturity. This has been accomplished by isopycnic sedimentation of marrow cells in density gradients of Ficoll and Hypaque under conditions in which erythrocyte precursors are virtually eliminated and lymphocyte contamination is small. Immature cells have lower buoyant density and predominate in the top of the gradients and cells of increasing maturity are found as the density level of the gradient increases. Distinct differences were found between control marrows and those of animals with an intense inflammatory response. The inflammatory stimulus depleted the marrow of many mature granulocytes and the immature cell populations were shown to be enriched. Peritoneal exudate cells (mostly mature granulocytes) were shown to have altered buoyant density properties compared to mature granulocytes in marrow. Activity of alkaline phosphatase (a granule‐associated enzyme) increased progressively as cells matured, while lactic dehydrogenase activity (a cytosol enzyme) remained stable. Protein synthesis in control and stimulated marrows showed two‐ to five‐fold greater synthesis in immature cells compared to nearly mature marrow cells. Successful separation of varying stages of development in granulocytes permits varied studies of cell properties involved in the process of differentiation.