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Intraerythrocyte pH, pCO 2 and the Hexose Monophosphate Shunt
Author(s) -
Warth James A.,
Desforges Jane F.,
Stolberg Sherry
Publication year - 1977
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1977.tb01008.x
Subject(s) - hexose , pentose phosphate pathway , chemistry , methanol , stimulation , shunt (medical) , biochemistry , pco2 , medicine , chromatography , glycolysis , enzyme , organic chemistry
S ummary . We have documented a rise in pCO 2 when erythrocytes are haemolysed by freeze‐thaw using solid CO 2 and methanol. This is partially corrected by preventing leakage of CO 2 into the system. The remaining increment is partially explained by stimulation of the hexose monophosphate shunt when erythrocytes are haemolysed by this technique.