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Increased Survival of Haemopoietic Pluripotent Stem Cells in Vitro Induced by a Marrow Fibroblast Factor
Author(s) -
Blackburn Mary J.,
Patt Harvey M.
Publication year - 1977
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1977.tb01004.x
Subject(s) - bone marrow , haematopoiesis , stem cell , biology , induced pluripotent stem cell , andrology , in vitro , fibroblast , immunology , microbiology and biotechnology , embryonic stem cell , medicine , biochemistry , gene
S ummary . When mouse bone marrow was incubated in medium conditioned by marrow fibroblasts, the survival of pluripotent stem cells (CFU S ) was considerably greater than when marrow was incubated in fresh medium. This increase in CFU S survival depended on the age of the marrow fibroblast culture, the initial number of cells in the culture, and the concentration of the conditioned medium. Medium conditioned by fibroblasts from other adult tissues—spleen, bone, and subcutaneous tissue—did not increase CFU S survival, but medium conditioned by embryo bone did. The increase in CFU S survival by marrow‐fibroblast‐conditioned medium was not accompanied by any change in the total number of nucleated cells of the incubated marrow nor by any comparable increase in the survival of granulopoietic stem cells (CFU C ) or erythropoietic stem cells (BFU E ). These results indicate that marrow fibroblasts produce a factor that increases the survival of CFU S , which may be involved in the role of marrow stroma in the control of haemopoiesis.