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Transferrin Receptors in Developing Murine Erythroid Cells
Author(s) -
Nunez M. T.,
Glass J.,
Fischer S.,
Lavidor L. M.,
Lenk E. M.,
Robinson S. H.
Publication year - 1977
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1977.tb00992.x
Subject(s) - transferrin , transferrin receptor , receptor , intracellular , microbiology and biotechnology , cell , biology , cell surface receptor , chemistry , biochemistry
S ummary . Techniques of cell separation were used to isolate murine erythroid cells at different stages of maturation. The number of transferrin receptors in these cell populations was assayed by measuring binding of 125 I‐labelled transferrin. Nearly 23 times as many receptors were found in the least mature cells, chiefly pronormoblasts, as in reticulocytes. Iron transport, determined by measurement of the rate of 59 Fe uptake from 59 Fe‐labelled transferrin, was proportional to the number of receptors at all stages of differentiation. Electron microscope radioautographic studies of the interaction of 125 I‐labelled transferrin with erythroid precursor cells demonstrated that 15–35% of cell associated transferrin was intracellular in erythroid precursors.