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Specific Interaction of HLA Antibodies (Eluates) with Washed Platelets
Author(s) -
Heinrich D.,
Stephinger U.,
Muellereckhardt C.
Publication year - 1977
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1977.tb00605.x
Subject(s) - platelet , chemistry , apyrase , fibrinogen , antibody , biochemistry , calcium , immunology , microbiology and biotechnology , enzyme , biology , organic chemistry
The mechanism of complement‐independent action of HLA‐A2 antibodies (eluates) on washed platelets was investigated. HLA‐specific alteration was confirmed by serological (platelet micro‐complement fixation), morphological (platelet spreading) and functional parameters (platelet aggregation, inhibition of collagen‐induced platelet aggregation, [ 14 C]serotonin release). In the presence of fibrinogen and calcium ions, HLA antibodies induced instantaneous platelet aggregation and release. Although no morphological (spreading) and functional changes (collagen‐induced aggregation) were seen, these platelets did not aggregate or release when fibrinogen was subsequently added. When platelets—in the presence of fibrinogen—were incubated with antibody concentrations too low to induce platelet aggregation or release, specific reduction of platelet reactivity was observed by subsequent collagen aggregation. HLA‐specific action of antibodies on washed platelets was inhibited by apyrase and acetyl‐salicylic acid, indicating an active participation of platelets in HLA antibody‐induced platelet alteration.