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Globin Synthesis in Normal Human Bone Marrow
Author(s) -
CHALEVELAKIS G.,
CLEGG J. B.,
WEATHERALL D. J.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb03600.x
Subject(s) - globin , sephadex , beta (programming language) , alpha (finance) , bone marrow , fractionation , chemistry , chromatography , beta globulins , biochemistry , in vitro , microbiology and biotechnology , biology , hemoglobin , gamma globulin , immunology , medicine , enzyme , antibody , construct validity , nursing , computer science , programming language , patient satisfaction
S ummary . Globin synthesis has been studied by in vitro labelling with radioactive amino acids in 60 normal human bone‐marrow samples. Under the conditions routinely used to fractionate α and β chains by chromatography α/β production ratios ranging from 0.5 to 1.0 were obtained, depending on the method of sample treatment. This variation was due entirely to the presence of non‐haem proteins derived from white cells which chromatograph with globin on CM‐cellulose. Purification of globin on Sephadex G100 and fractionation of α and β globin chains by a modified chromatographic system resulted in α/β ratios of unity. The relevance of these findings to the study of marrows in which there is unbalanced globin chain production is discussed.