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Preparation and Storage of Platelet Concentrates: I. FACTORS INFLUENCING THE HARVEST OF VIABLE PLATELETS FROM WHOLE BLOOD
Author(s) -
SLICHTER S. J.,
HARKER L. A.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb03586.x
Subject(s) - platelet , centrifugation , platelet concentrate , whole blood , pellet , chemistry , platelet rich plasma , blood component , andrology , chromatography , food science , medicine , immunology , biology , intensive care medicine , zoology
S ummary . Factors affecting the yield and viability of concentrated platelets have been investigated in a blood component programme. It was found that 86± 1% of the platelets from a unit of whole blood can be concentrated without loss of viability by processing ACD or CPD anticoagulated blood at room temperature. The steps are initial centrifugation at 1000 g for 9 min to harvest platelet‐rich plasma; centrifugation of the platelet‐rich plasma at 3000 g for 20 min to pack the platelets; and resuspension of the platelet pellet in residual plasma after 11/2 h.