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In Vivo Plasma and Urine Folate Binding after Ingestion of 3 H‐Folic Acid and 14 C‐Methyl‐Folate
Author(s) -
Retief F. P.,
Heyns A. du P.,
Oosthuizen Mariëtha,
Reenen O. R.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb03559.x
Subject(s) - sephadex , chemistry , urine , ingestion , in vivo , elution , folic acid , chromatography , biochemistry , medicine , enzyme , biology , microbiology and biotechnology
After simultaneous ingestion of equivalent amounts of [ 3 H]folic acid ( 3 H‐PteGlu) and [ 14 C]N 5 ‐methyl‐tetrahydrofolic acid ( 14 C‐CH 3 ‐H 4 PteGlu) we were able to demonstrate progressive macromolecular binding of radiofolate in plasma, which appeared to be near maximal at 6 h. Bound radiofolate was predominantly of 14 C‐CH 3 H 4 PteGlu origin, and only at 24 h could 3 H incorporation be demonstrated. The binder eluted with albumin from Sephadex DEAE‐A50 columns. In urine a smaller bound radiofolate fraction, with approximately equal amounts of 3 H and 14 C, appeared after 5.5 h. Plasma chromatography showed radio‐PteGlu (peak 1) to be rapidly converted to CH 3 ‐H 4 PteGlu (peak 2), with subsequent appearance of two further radiofolate peaks (peaks 3 and 4) the nature of which is as yet unclear. Urine showed similarly placed fractions but their magnitude differed, and urinary peak 3 in particular was much more prominent than its plasma counterpart.