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Haemopoietic Progenitor Cells in Prenatal Congenitally Anaemic ‘Flexed‐Tailed’ ( f/f ) Mice
Author(s) -
Cole R. J.,
Regan T.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb03555.x
Subject(s) - fetus , congenic , biology , spleen , andrology , progenitor cell , erythropoiesis , immunology , gestation , microbiology and biotechnology , medicine , endocrinology , stem cell , pregnancy , anemia , genetics , gene
S ummary . The incidences of erythroid colony forming cells (CFU e ) and granulocyte‐macrophage colony forming cells (CFU C ) have been measured in 11–18 d prenatal livers of mice of genotype f/f and nearly congenic +/+ controls. In normal fetal livers numbers of CFU e (cells able to form colonies of 16 or more cells after 72 h in vitro ) rise to a maximum on day 14 of gestation and represent c 1% of total fetal liver cells. In f/f fetal livers, peak values for numbers and proportions of CFU e are 50% of normal. They f/f lesion does not reduce the numbers of CFU c in fetal liver. Since this deficiency in CFU e parallels deficiencies of similar magnitude in spleen‐colony forming units (CFU s ) and erythroblasts in the liver, and erythrocytes in the blood, of f/f fetuses it is concluded that the f/f lesion is expressed at an early stage of haemopoietic development in prenatal life. The possibility that restricted haem synthesis is the primary effect of the f/f genotype and responsible for disturbances of both haemopoietic cellular proliferation and haemoglobin synthesis is examined.