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Nuclear DNA‐Polymerase Estimation in Human Leukaemic Myeloblasts
Author(s) -
Wantzin Gunhild Lange,
Karle Hans,
Killmann SvenAage
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb03548.x
Subject(s) - dna polymerase , microbiology and biotechnology , polymerase , primer (cosmetics) , thymidine , dna , cytosine , polymerase chain reaction , dna synthesis , cytarabine , chemistry , biology , biochemistry , cancer research , myeloid leukemia , gene , organic chemistry
S ummary . A technique which detects the presence of DNA‐polymerase in cell nuclei by measuring the incorporation of 3 H‐thymidine‐5‐triphosphate ( 3 H‐TTP) has been used to estimate the proportion of leukaemic myeloblasts which contains DNA‐polymerase and DNA capable of acting as primer‐template. In six cases of previously untreated acute myeloid leukaemia the 3 H‐TTP labelling index ( 3 H‐TTP LI) was much larger than the fraction in DNA synthesis. After a single ‘flash’ injection of cytosine arabinoside a pronounced decline was observed in the 3 H‐TTP LI, which can be explained by a direct inhibition of DNA‐polymerase. No change was observed in the fraction of cells labelled with 3 H‐thymidine. A decrease in 3 H‐TTP LI was also observed after a single i.v. dose of methotrexate.