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Surface Sulphydryl Groups and Hexose Monophosphate Pathway Activity in Resting Human Polymorphonuclear Leucocytes
Author(s) -
Tsan MinFu,
Newman Burlina,
Chusid Michael J.,
Wolff Sheldon M.,
McIntyre Patricia A.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb03531.x
Subject(s) - concanavalin a , taurocholic acid , hexose , stimulation , chemistry , biochemistry , medicine , endocrinology , enzyme , biology , in vitro , bile acid
S ummary . The role of surface ‐SH groups on the hexose monophosphate pathway activity in resting human polymorphonuclear leucocytes was studied. Endotoxin, taurocholic acid and concanavalin A stimulated [1‐ 14 C]glucose oxidation to a greater degree than [6‐ 14 C]glucose oxidation. The stimulation of glucose‐1‐C oxidation by endotoxin, taurocholic acid and concanavalin A could be prevented by p ‐chloromercurybenzene sulphonic acid, an inhibitor of surface ‐SH groups. In contrast, polymorphonuclear leucocytes from four patients with chronic granulomatous disease failed to show stimulation of glucose‐1‐C oxidation by endotoxin, taurocholic acid or concanavalin A.