Premium
The Diurnal Rhythm of Enzymes in Human Red Cells
Author(s) -
BrokSimoni Frida,
Ashkenazi Yisrael E.,
Ramot Bracha,
Holtzman Fanny
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb00964.x
Subject(s) - oxidoreductase , dehydrogenase , lactate dehydrogenase , circadian rhythm , biochemistry , nad+ kinase , biology , hexokinase , enzyme , medicine , endocrinology , microbiology and biotechnology , glycolysis
The activities of glucose‐6‐phosphate dehydrogenase (D‐glucose‐6‐phosphate: NADP oxidoreductase, G6PD), 6‐phosphogluconate dehydrogenase (6‐phospho‐d‐gluconate: NADP oxidoreductase, 6PGD), hexokinase (ATP: D‐hexose 6‐phosphotransferase, HK), lactic dehydrogenase (L‐lactate: NAD oxidoreductase, LDH) and aspirate aminotransferase (L‐aspartate: 2‐oxoglutarate aminotransferase, Asp. T) were determined in red blood cells of 11 healthy individuals. The determinations were carried out on samples drawn every 4 h over a 24 h period. The activities of G6PD, 6PGD, LDH and Asp. T exhibited a semi‐circadian rhythm, namely, two peaks of activity during 24 h while HK activity demonstrated a true circadian rhythm. In addition a polymorphism of the G6PD and LDH activity patterns was observed. The implications of a biological clock in enucleated cells are discussed.