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Measurement of the Mean Cell Volume using Electronic Particle Counters
Author(s) -
England J. M.,
Down M. C.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb00944.x
Subject(s) - coulter counter , centrifugation , nuclear medicine , blood cell , mean difference , red blood cell , red cell , physics , statistics , medicine , immunology , chemistry , mathematics , chromatography , microbiology and biotechnology , biology , confidence interval
Under carefully controlled conditions electronic cell counters, for example the Coulter Counter, Model F N , and Channelyzcr, may be calibrated to give MCV values down to as small as 20 fl which agree with those derived from the centrifugation PCV (corrected for plasma trapping) and the red cell count. The MCV values will be too high if the instrument uses a high cell concentration, has a fixed lower threshold, no effective upper threshold and no edit facility. This may partly explain why the Coulter Counter, Model S, when standardized with 4C cell control gives higher MCV values than the Model F N linked to the Channelyzer. The difference is, on average, 2 fl with normal blood samples and 5 fl in cases of microcytic anaemia. It is suggested that standards of low MCV should be used together with those of normal MCV when calibrating the Model S.