Premium
Haemolytic Effect of Two Sulphonamides Evaluated by a New Method
Author(s) -
Gaetani G. D.,
Mareni C.,
Ravazzolo R.,
Salvidio E.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb00921.x
Subject(s) - haemolysis , chloroquine , glucose 6 phosphate dehydrogenase , pentose phosphate pathway , dehydrogenase , pharmacology , incubation , biology , oxidoreductase , stimulation , biochemistry , chemistry , enzyme , medicine , endocrinology , immunology , glycolysis , malaria
A technique to investigate drugs which could cause haemolysis in subjects deficient in glucosc‐6‐phosphate dehydrogenase (D‐glucose‐6‐phosphate: NADP oxidoreductase; G6PD) has been developed. The method is based on the technique of 14 CO 2 evolution during the incubation of normal erythrocytes in the presence of [I‐ 14 C] glucose and their own serum, the latter containing the active metabolites of the drugs received by normal subjects. By this method agents causing a stimulation of the hexosemonophosphate pathway of normal erythrocytes should be regarded as potentially haemolytic for G6PD‐deficient subjects. Two sulphonamides, sulphormethoxine and sulphalene, of which until now no haemolytic effects have been reported, together with chloroquine, have been investigated. While chloroquine does not affect the hexosemonophosphate shunt of normal erythrocytes, the two sulphonamides stimulate this pathway. The results are confirmed by the reduction of the half‐life of 51 Cr‐labelled G6PD‐deficient red cells (Mediterranean variant), after administration of the two sulphonamides.