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Factor VIII and Human Platelet Aggregation
Author(s) -
Bottecchia D.,
Vermylen J.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb00201.x
Subject(s) - apyrase , chemistry , platelet , biochemistry , serotonin , platelet factor 4 , platelet rich plasma , extracellular , immunology , heparin , biology , receptor
SUMMARY Bovine factor VIII aggregates human platelets either in a strong single wave at high concentration (10 ug/ml platelet suspension) or in two waves at low wave at low concentration 2–1 ug/ml). The strong single wave of aggregation is not associated of [ 14 C]serotonin or β‐glucuronidase; the high concentration retraction of reptilase‐clotted platelet‐rich plasma. With the low release of [ 14 C]serotonin is observed just prior to the onset of the aggregation; release of β‐glucuronidase does not occur at any low concentration of bovine factor VIII induces moderate retraction of platelet‐rich plasma, which is inhibited by acetylsalicylic acid, in and apyrase, indicating that it is a consequence of release of platelet phosphate. It has previusly been suggested that carbohydrate groups are involved in the bovine factor VIII interaction, since galactose oxidase and periodate the platelet aggregating activity of bovine factor VIII. The present these oxidizing substances also induce a degradation of bovine the exact role of carbohydrate groups in the aggregation process established.