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Factor VIII and Human Platelet Aggregation
Author(s) -
Bottecchia D.,
Vermylen J.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb00200.x
Subject(s) - platelet , platelet aggregation , medicine , chemistry
SUMMARY Bovine factor VIII is a potent inducer of aggregation of human platelets. Upon gel filtration of five‐thousand‐fold purified material in 0.5 M CaCl 2 , bovine factor VIII is separated into high and low molecular weight components: the former contains both a ‘carrier protein’and platelet aggregating activity, the latter theproco‐agulant activity (low molecular weight factor VHI, LMW‐F VIII). Upon removal of Ca 2+ ions, LMW‐F VIII recombines with the ‘carrier protein’. LMW‐F VIII modifies aggregation by ‘carrier protein’, but not aggregation by undissociated bovine factor VIII, adenosine‐5′‐diphosphate or adrenaline. This finding indicates that the platelet aggregating activity is indeed a property of the ‘carrier protein’.