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Activation of Platelets by Platelet Activating Factor (PAF) Derived from IgE‐Sensitized Basophils
Author(s) -
Henson Peter M.,
Landes Richard R.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb00197.x
Subject(s) - platelet , secretion , platelet activating factor , chemistry , immunoglobulin e , platelet activation , thrombin , granule (geology) , platelet factor 4 , platelet aggregation , immunology , endocrinology , medicine , microbiology and biotechnology , biochemistry , biology , antibody , paleontology
SUMMARY Platelet activating factor (PAF) derived from antigen‐stimulated, IgE‐sensitized rabbit basophils acts on platelets to induce aggregation and secretion of their content of granule‐bound vasoactive amines. Despite this, PAF did not activate platelet factor 3. In contrast, collagen induced aggregation, secretion and PF 3 activation in the washed platelets. Other stimuli (ADP, C 3 b, thrombin) also initiated both secretion and PF 3 activation. A wide dose range of PAF, including those giving maximal secretion and aggregation, were ineffective in making PF 3 available and the possibility that PAF inhibited PF 3 , or its generation, was also excluded. It is concluded that PAF is a unique stimulus for platelets and that secretion and aggregation are not necessarily accompanied by PF 3 generation.