Premium
Blood Leucocyte Enzymes: III. DIURNAL RHYTHM OF ACTIVITY IN ISOLATED LYMPHOCYTES OF NORMAL SUBJECTS AND CHRONIC LYMPHATIC LEUKAEMIA PATIENTS
Author(s) -
Ramot Bracha,
BROKSIMONI Frida,
Chweidan Emma,
Ashkenazi Yisrael E.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb00176.x
Subject(s) - medicine , lymphatic system , peripheral blood , hematology , enzyme , immunology , pathology , biology , biochemistry
SUMMARY. The activities of glucose‐6‐phosphate dehydrogenase (D‐glucose‐6‐phosphate: NADP oxidoreductase, G6PD), 6 phosphate glucono dehydrogenase (6 phospho‐D‐gluconate: NADP oxido reductase, 6PGD) lactate dehydrogenase (D‐lactate: NAD oxidoreductase, LDH), glutamate oxaloacetate transaminase (L‐aspartate: 2‐oxo‐glutarate aminotransferase, GOT) and hexokinase(ATP: D‐hexo‐6‐phosphotrans‐ferase, Hx) were measured over 24 h in isolated lymphocytes of normal subjects and in white cells of patients with chronic lymphatic leukaemia (CLL). The activity patterns of all enzymes in the normal lymphocytes were similar. A computed pattern of all the results exhibited a circadian rhythm of activity with the highest level at 16.00 hours. The oscillations in the activities of the same enzymes in the CLL cells differed among the patients, although all the enzymes of the same individual showed a similar diurnal rhythmic pattern. All peaks in this group appeared between 20.00 and 08.00 hours. The possible importance of these observations in setting up therapeutic schedules was raised.