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Kinetic Studies of 51 Cr and DF 32 P Labelled Granulocytes
Author(s) -
Dresch C.,
Najean Y.,
Bauchet J.
Publication year - 1975
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1975.tb01800.x
Subject(s) - granulocyte , ficoll , microbiology and biotechnology , isotopes of chromium , immunology , radiochemistry , chemistry , in vitro , medicine , biology , biochemistry , peripheral blood mononuclear cell
S ummary .In vivo kinetic studies of granulocytes labelled in vitro with 51 Cr and DF 32 P were carried out in nine haematologically normal subjects by isolation of the cells in the blood samples by the Ficoll‐Isopaque flotation method. 51 Cr and 32 P specific activity of blood samples made of 93–98% granulocytes was studied. Distribution between marginated and circulating granulocyte pools was identical for both labelled cells and the marginated pool was similar to the circulating pool, except that it was lower in one subject who had a previous splenectomy. The half‐disappearance time ( T 1/2) was 16.1 ± 2.2 h for 51 Cr‐labelled and 5.4±2.1 hr for DF 32 P‐labelled granulocytes. In one case of a normal subject who previously received multiple transfusion homologous 51 Cr‐labelled granulocytes had a T 1/2 of less than I h.