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Quantitation of Subclasses of Mononuclear Cells in Normal Human Blood by Membrane Receptor Studies
Author(s) -
Habeshaw J. A.,
Young Gillian A.
Publication year - 1975
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1975.tb01798.x
Subject(s) - peripheral blood mononuclear cell , receptor , cell , antibody , blood cell , chemistry , human blood , immunology , cell surface receptor , microbiology and biotechnology , biology , in vitro , biochemistry , physiology
S ummary . A technique for quantitating mononuclear cells expressing E, EA (IgG) , EAC receptors, immunoglobulin carrying cells and phagocytic cells in normal human venous blood is described; normal values for each of these classes of mononuclear cell are established. The effects of different methods of cell preparation have been considered: these were found not to have a significant effect on the final values. In addition, we describe a second category of ‘fluorescent non‐capping’ cells and show that these are numerically equivalent to cells which ingest neutral red.