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Elevated Microviscosity in Membranes of Erythrocytes Affected by Hereditary Spherocytosis
Author(s) -
Aloni B.,
Shinitzky M.,
Moses S.,
Livne A.
Publication year - 1975
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1975.tb00839.x
Subject(s) - microviscosity , hereditary spherocytosis , erythrocyte fragility , membrane , chemistry , fluorescence anisotropy , membrane fluidity , red blood cell , biophysics , microbiology and biotechnology , biochemistry , hemolysis , medicine , biology
S ummary . Erythrocytes affected by hereditary spherocytosis (HS), obtained from several splenectomized patients, showed a varying degree of elevated osmotic fragility. In order to evaluate a possible role of the crythrocyte membrane lipids in HS, microviscosity of the membrane lipid core was measured by a fluorescence‐polarization technique. Intact HS‐affected red cells, as well as their ghost membranes and liposomes prepared from their lipid extract, all showed a distinctly higher microviscosity than the respective normal control. The increased microviscosity correlated with the severity of HS. The data support the proposition that the defect in HS‐affected red cells is associated, at least in part, with alterations in the membrane lipids.