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In Vitro Labelling of Platelets: an Experimental Study on Healthy Asplenic Subjects using Two Different Incubation Media
Author(s) -
Kutti Jack,
SafaiKutti Soodabeh
Publication year - 1975
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1975.tb00832.x
Subject(s) - platelet , incubation , labelling , sodium citrate , isotopes of chromium , chemistry , medicine , endocrinology , pathology , biochemistry
S ummary . Duplicate platelet survival studies, using autologous platelets labelled in vitro with radioactive sodium chromate, were performed on six young healthy asplenic men. In the first experiment plasma, and in the second a Ringer‐citratedextrose (RCD) solution (Abrahamsen, 1968), was employed as incubation medium. The uptake of chromate by the platelets was about 2.5 times higher in the RCD compared to the plasma experiments. An identical pattern for the immediate behaviour of infused labelled platelets was observed in the duplicate studies, and the recovery of platelet‐bound radioactivity remained stable at the 90% level during 2 h post‐infusion. In these experiments the means for platelet mean life span (MLS) were identical, 7.2 ± 0.5 and 7.2±0.4 days, respectively. These values slightly, but not significantly, exceeded the mean platelet MLS for a control group consisting of 10 young healthy males (6.9±0.4 days). The means for platelet production rate (P) at the duplicate studies made on the asplenic subjects were 19±2 × 10 10 and 21±2 × 10 10 platelets per day, respectively, and did not differ from the mean for P obtained in the control‐group (22±2 × 10 10 ).