Premium
A Comparison between Radioimmunoassay and Other Immunological Techniques for the Measurement of Fibrinogen/Fibrin Degradation Products in Serum
Author(s) -
Ratky Susan M.,
Martin Marion J.,
Gordon Y. B.,
Baker L. R. I.,
Leslie J.,
Chard T.
Publication year - 1975
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1975.tb00528.x
Subject(s) - radioimmunoassay , immunoassay , latex fixation test , chemistry , fibrinogen , chromatography , agglutination (biology) , fibrin , hemagglutination assay , hemagglutination , turbidimetry , biochemistry , antibody , immunology , medicine , titer
S ummary . Fibrinogen/fibrin degradation products (FDP) have been measured in serum by radioimmunoassay for fragment E (FgE), one of the terminal fragments in plasmic digestion of fibrinogen, and the results compared with those determined by both a tanned red cell haemagglutination inhibition immunoassay (TRCHII) and a latex particle agglutination inhibition immunoassay. The detection limit of the FgE assay was 0.8 ng/ml, that of TRCHII was 625 ng/ml and the latex particle agglutination inhibition immunoassay was 10 μg/ml. All the samples measured had detectable levels of FDP with the FgE assay, whereas only 88% were measurable with the TRCHII and 2% by the latex particle agglutination inhibition immunoassay. A comparison of those samples giving a positive result with both the TRCHII and FgE assay showed overall agreement between the results of the two types of assay, but there was considerable scatter of FgE levels at each point of the TRCHII. The major advantages of the radioimmunoassay system are greater sensitivity, specificity and reproducibility.