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Quantitation of the ABO System Antigens by Means of Isoprecipitating Antisera
Author(s) -
Pintera J.,
Vacl J.,
Hrnčířová D.,
Kurtinová J.,
Zelená E.
Publication year - 1975
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1975.tb00520.x
Subject(s) - abo blood group system , antiserum , population , antigen , antibody , red cell , blood group antigens , chromatography , saliva , chemistry , titration , homogeneity (statistics) , biology , immunology , medicine , biochemistry , mathematics , inorganic chemistry , statistics , environmental health
S ummary . A quantitative method for the determination of blood group specific substances A and B on red blood cells was developed, using them to absorb isoprecipitating antisera anti‐A and anti‐B, followed by estimation of the residual antibody by means of a counterimmunoelectrophoretic technique with standard solutions of A and B substances prepared from saliva. The method was tested on a population of A 2 B donors. The mean amount of A substance in A 2 B RBC was 131 × 10 −12 ±217 × 10 −12 u/cell in the population of 95 donors. The A substance thus determined agreed significantly with values obtained by classical haemagglutinating titration. The mean amount of B substance in A 2 B RBC was 222 × 10 −12 ±178 × 10 −12 u/cell. The amounts of both the A and B substances were not correlated on the same RBC. Several peaks of the distribution curve of both substances confirmed the nonhomogeneity of the populations, there being a wide standard deviation presumably due to differences in the antigen content of different subgroups.