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In Vitro Stimulation of Chronic Lymphocytic Leukaemic Lymphocytes
Author(s) -
Perera D. J. B.,
Pegrum G. D.
Publication year - 1974
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1974.tb06795.x
Subject(s) - phytohaemagglutinin , pokeweed mitogen , concanavalin a , stimulation , in vitro , lymphocytosis , population , immunology , lymphocyte , microbiology and biotechnology , chemistry , biology , endocrinology , medicine , biochemistry , environmental health
S ummary . The response of chronic lymphocytic leukaemic (CLL) lymphocytes to various mitogens in 5‐day cultures has been studied. Concanavalin A (Con.A) and pokeweed mitogen (PWM) were not superior to phytohaemagglutinin (PHA) in their stimulatory activity. Suboptimal levels of PHA and PWM together caused greater RNA synthesis than either mitogen at their optimal levels. The response to PHA could be chanced by the addition of a specific number of red blood cells and by glutathione. Using transformed nuclear material a reaction approaching the level found in normal lymphocytes was attained clearly indicating marked stimulation of the leukaemic cell population.