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Endocytosis and Exocytosis in Membrane Remodelling during Reticdocyte Maturation
Author(s) -
Gasko O.,
Da D.
Publication year - 1974
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1974.tb06665.x
Subject(s) - exocytosis , endocytosis , pinocytosis , microbiology and biotechnology , intracellular , chemistry , vesicle , organelle , reticulocyte , membrane , biophysics , cell , biology , biochemistry , rna , gene
S ummary . Young reticulocytes have a greater surface area and surface charge density than mature reticulocytes. Membrane remodelling of maturing reticulocytes was investigated by electron microscopic methods. Endocytosis was demonstrated in reticulocytes by a‘two stage labelling’technique in which colloidal gold particles were used as a substrate for pinocytosis; after fixation, cationized ferritin served as a marker for the membrane connected with the outer cell surface. Incubation of reticulocytes in agar semi‐solid media, which prevents subcellular elements from migrating, demonstrated exocytosis and shedding of membranous structures and cell fragments. Membrane remodelling of the reticulocyte appears to occur by internali‐zation of the plasmalemma. This is followed either by intracellular digestion or by exocytosis with previously existing vesicles, as well as by fragmentation and detachment of microspheres containing haemoglobin.