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The Radioimmunoassay of Serum and Salivary Blood Group A and Le a Glycoproteins
Author(s) -
Houlburn A. M.,
Masters Carole A.
Publication year - 1974
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1974.tb06650.x
Subject(s) - radioimmunoassay , abo blood group system , saliva , glycoprotein , antigen , chemistry , microbiology and biotechnology , immunology , biology , biochemistry
S ummary . A radioimmunoassay was developed for the detection of A and Le a blood group activities in glycoprotein fractions extracted with perchloric acid (PCA) from normal serum. The technique was also applied to studies of salivary blood group glycoproteins. Blood group A activity was detected in all group A sera tested. Higher levels of A activity were found in sera from group A 1 than from A 2 donors, and sera from both group A 1 and A 2 secretor donors gave higher levels of A activity than sera from non‐secretor donors. No difference was found in the levels of A activity in group A 1 and A 2 secretor salivas but only small numbers were assayed. Parallel radioinimunoassay inhibition lines, indicating immunological identity, were obtained with PCA‐extracts of A 1 and A 2 secretor plasma and with extracts of A 1 and A 2 secretor salivas. Le a activity was detected in all sera tested. The levels of Le a activity were higher in sera from Le(a + b ‐) donors than from h(a ‐ b +) or Le(a ‐ b ‐) donors and were unaffected by ABO group. Radioimmunoassay inhibition lines indicated qualitative differences in the Le a antigen in salivas from secretors and non‐secretors, consistent with a lower density of Le a antigen on salivary glycoprotein from secretor donors.