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Effect of Heterologous Anti‐lymphocyte and Anti‐granulocyte Sera on Haematopoietic Progenitor Cells in the Rat
Author(s) -
Benestad H. B.,
Iversen J.G.,
Warhuus K.
Publication year - 1974
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1974.tb00816.x
Subject(s) - antiserum , progenitor cell , haematopoiesis , heterologous , biology , bone marrow , granulocyte , immunology , microbiology and biotechnology , lymphocyte , lymph , peripheral blood mononuclear cell , andrology , pathology , stem cell , antibody , medicine , in vitro , biochemistry , gene
S ummary . The effect on haematopoietic progenitor cells of specific antisera against mature haemic cells has been investigated. Heterologous anti‐lymphocyte and anti‐granulocyte sera were prepared by injecting suspensions of rat target cells into rabbits. Recirculating lymphocytes were obtained from thoracic duct lymph, non‐recirculating ones from blood of rats in which thoracic duct lymph had been drained away for 3 days, and mature granulocytes were isolated from blood of normal rats. Preliminary assaying of the cytotoxic activity of the sera was done by measuring isotope release from target cells proper, labelled with 51 Cr. When absorption procedures had been carried out the antisera were apparently specific for recirculating lymphocytes, nonrecirculating lymphocytes, or granulocytes. The effect of these antisera on progenitor cells for granulocytes and on mature cell types were then evaluated with the diffusion chamber culture technique. Mononuclear cells from rat blood, bone marrow cells, or a mixture of both were incubated with different dilutions of antisera before culturing for I or 4 days. The yield of the cell types used for immunization was reduced with all employed dilutions of antisera. The number of proliferative granulocytes harvested from 4‐day cultures was taken to reflect the number of progenitors inoculated. Anti‐recirculating lymphocyte sera never affected progenitor cells, and they could be spared with some dilutions of antisera against nonrecirculating lymphocytes and granulocytes. It is thus possible to prepare specific antisera against lymphocytes with no harmful effect on progenitor cells.