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Absence of B‐ and T‐Cell Markers on Acute Lymphoblastic Leukaemic Cells and Persistence of the T‐Cell Marker on Mitogen‐Transformed T‐Lymphocytes
Author(s) -
Collins R. D.,
Smith L.,
Clein G. P.,
Barker C. R.
Publication year - 1974
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1974.tb00505.x
Subject(s) - lymphoblast , surface immunoglobulin , biology , microbiology and biotechnology , immunology , bone marrow , mitosis , cell , b cell , antibody , cell culture , genetics
S ummary . Lymphocytes from six patients with acute lymphoblastic leukaemia (ALL) were studied in vitro for thymus (T‐cell) and bone marrow‐derived (B‐cell) markers. Leukaemic lymphoblasts did not form rosettes with sheep erythrocytes (T‐cell marker) or bear surface immunoglobulin determinants by indirect immuno‐fluorescent staining (B‐cell marker). The failure of lymphoblasts to form rosettes was apparently not due to the fact that lymphoblasts were in various stages of the cell cycle, as mitogen‐transformed T‐cells and T‐cells in mitosis retained their capacity to form rosettes with sheep erythrocytes. In contrast, leukaemic B‐cells, obtained from a patient with chronic lymphocytic leukaemia and recognized by intracellular immunoglobulin crystals, did not form rosettes nor did a small number of mitogen‐transformed leukaemic cells. These studies suggest that lymphoblasts from some patients with the haematological picture of ALL do not have surface markers for either T‐ or B‐cells.