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Haemoglobin Synthesis in Suspension Cultures of Human Bone Marrow
Author(s) -
Wood W. G.
Publication year - 1974
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1974.tb00486.x
Subject(s) - bone marrow , erythropoietin , in vitro , suspension culture , globin , erythropoiesis , microbiology and biotechnology , immunology , biology , hemoglobin , endocrinology , cell culture , andrology , chemistry , medicine , pathology , anemia , biochemistry , genetics
S ummary . The pattern of haemoglobin and individual globin chain synthesis was examined in suspension cultures of adult human bone marrow. Haemoglobin A synthesis continued as long as normoblasts remained in culture and was not observed after their disappearance. The addition of erythropoietin increased and prolonged haemoglobin synthesis up to 12 days, an effect which showed good correlation with the numbers of immature erythroid cells present in culture. Previous reports of Hb F and γ chain synthesis by adult marrow in vitro could not be confirmed.