Premium
Renal Mechanisms Underlying Cyclic AMP Action on Erythropoiesis
Author(s) -
Peschle Cesare,
Rappaport Ira A.,
D'Avanzo Antonio,
Russolillo Silvia,
Marone Gianni,
Condorelli Mario
Publication year - 1973
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1973.tb01750.x
Subject(s) - erythropoiesis , endogeny , endocrinology , medicine , dbc , erythropoietin , chemistry , in vivo , mechanism of action , stimulation , biology , in vitro , anemia , biochemistry , physics , microbiology and biotechnology , phase noise , optics
S ummary . Dibutyryl cyclic AMP (dbc‐AMP) was injected into ex‐hypoxic polycythaemic mice either alone or with anti‐erythropoietin (anti‐Ep) serum. Anti‐Ep totally abolishes the wave of erythropoiesis evoked by dbc‐AMP. These results might indicate either that the action of this agent is totally Ep‐dependent, or that a residual amount of endogenous Ep is necessary to allow dbc‐AMP to exert a direct effect at the marrow level. The latter mechanism, however, is precluded by experiments indicating that administration of moderate amounts of anti‐Ep, although abolishing totally the erythroid response to dbc‐AMP, do not induce complete suppression of endogenous Ep activity and erythropoiesis. Furthermore, a significant rise of Ep plasma levels is observed in rats receiving dbc‐AMP. Since this agent does not apparently modify the kinetics of endogenous Ep, it is postulated that dbc‐AMP induces a rise in Ep production. This phenomenon, although unmodified in ureter‐ligated animals, is completely abolished by bilateral nephrectomy. It is therefore concluded that dbc‐AMP induces in vivo a stimulatory effect on erythropoiesis via increased production of Ep, via a renal mechanism possibly represented by elevated levels of the renal erythropoietic factor.