Premium
Evaluation of 51 Cr as a Leucocyte Label
Author(s) -
Spivak Jerry L.,
Perry Seymour
Publication year - 1973
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1973.tb01743.x
Subject(s) - isotope , liquid scintillation counting , peripheral blood , radiochemistry , chemistry , nuclear medicine , microbiology and biotechnology , immunology , medicine , biology , physics , quantum mechanics
S ummary . A double label technique has been developed to compare the behaviour of 51 Cr labelled leucocytes with DF 32 P labelled leucocytes. Whole blood is divided into two aliquots and the leucocytes in each are labelled with a different isotope. After infusion of labelled cells, the radioactivity of each isotope can be determined simultaneously on the same blood sample using liquid scintillation counting. In studies of seven patients with solid tumours and one with leukaemia, the intravascular disappearance of autologous leucocytes labelled with 51 Cr and DF 32 P appeared similar in five of the eight patients during the initial 6 hr following infusion. Thereafter in all but one instance, 51 Cr labelled leucocytes had slightly higher specific activities. 51 Cr labelled lymphocytes and DF 32 P labelled lymphocytes also behaved in the same manner. It is concluded that 51 Cr is a suitable leucocyte label and may be substituted for DF 32 P under certain circumstances.